EXHIBIT 16 ---------- February 8, 2006 Securities and Exchange Commission 100 F Street, N.E. Washington, D.C. 20549 Gentlemen: We have read Item 4.01 of Network-1 Security Solutions, Inc.'s Form 8-K dated February 2, 2006, a copy of which the Registrant provided to us on February 8, 2006. We are in agreement with the statements regarding our firm in paragraph 1, sentence 1 and paragraph 2 of that item. We have no basis to agree or disagree with respect to any other information contained in Item 4.01. We did not conduct a review of the financial statements of Network-1 Security Solutions, Inc. for any period subsequent to September 30, 2005. Very truly yours, /s/ Eisner LLP - ------------------------- Eisner LLP